Sacramento-Yolo Counties Lyme Disease Surveillance 2005

Annual Report 2005, Sacramento-Yolo Mosquito and Vector Control District

Summary of Sacramento-Yolo MVCD 2004—2005 Tick Surveillance Data

Location          Total   Pools    Positive   Estimated Minimum
                  Ticks   Tested   Pools      Infection Rate
Yolo
Cache Creek        257      23         3              1.2%
Sacramento
Ancil Hoffman        0       0         0              N/A
Mississippi Bar     66      12         1              1.5%
Negro Bar           19      13         0              0.0%
Nimbus Dam          38       9         2              2.7%
Snipes Park         68      13         0              0.0%
Willow Creek        46      10         0              0.0%
Total              494      80         8

The bacterium that causes Lyme disease is called Borrelia burgdorferi. The primary vector for Lyme disease for Sacramento and Yolo Counties is Ixodes pacificus, also known as the western black—legged tick. The table... summarizes the Lyme disease surveillance data of Ixodes pacificus ticks collected from November 2004 through May 2005.

Tick specimens were collected by dragging a flannel sheet along the side of a trail, a technique called flagging. The ticks were pooled and tested by indirect immunofluorescence assay (IFA). A sample was considered positive by IFA if one or more fluorescently stained spiral—shaped bacteria were present. Some positive samples were confirmed using a polymerase chain reaction assay and by culture in liquid media.